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91.
P. R. Sharma S. Jain R. N. K. Bamezai P. K. Tiwari 《Indian journal of clinical biochemistry : IJCB》2010,25(1):57-63
The present study was carried out in the Sahariya tribe of Central India, which reportedly have high prevalence of pulmonary
tuberculosis. Total serum LDH and its tissue specific isoforms were estimated in TB patients and matched healthy controls
to test the utility of LDH as diagnostic marker for tuberculosis. About 210 sputum positive cases and 328 age and sex matched
sputum negative controls were recruited. The spectrophotometeric and densitometric analysis of each LDH isoform was carried
out in both cases and controls. The mean values of serum LDH were estimated and compared for each class by t-test. The statistical
comparisons were made between sputum negative controls and sputum positive cases by Mann-Whitney’s U test. The spectrophotometric
estimation of serum LDH revealed significant (P=0.0016) increase in its level in cases (290 IU/L) as compared to controls
(248 IU/L). The densitometric analysis of individual LDH isoforms in cases and controls demonstrated significant elevation
in LDH1 (P>0.05), LDH2 (P>0.05) and LDH3 (P<0.005) in sputum positive cases in comparison to sputum negative controls. Our
study revealed a positive correlation between serum LDH level and the presence of mycobacteria and their load, suggesting
utility of LDH as an important diagnostic marker of tuberculosis induced stress, at least in tribal areas lacking access to
modern clinical tests. 相似文献
92.
Pradyumna Kumar Mishra A. Bhargava R. P. Punde N. Pathak P. Desikan A. Jain S. Varshney K. K. Maudar 《Indian journal of clinical biochemistry : IJCB》2010,25(2):158-163
The present study included three groups: (A) age and gender matched control (n=24) with no previous signs of M. tuberculosis
complex (MTBC) infection, (B) patients (n=28) diagnosed with gastro-intestinal TB (GITB), (C) patients (n=50) with clinical
and histo-pathological signs of GITB, but were culture and AFB negative. Real time assay performed using fluorescence resonance
energy transfer hybridization probes showed a positivity index of 36 % in group C, i.e. 18 were found reactive from the total
50 cases studied. In addition, immune characterization of these 18 cases showed depleted CD4+ count and increased levels of IFN-γ and TNF-α cytokines. No positive case was found in group A, while in group B, out of
total 28 cases studied 27 were found positive. A combinatorial diagnostic approach for rapid detection and characterization
of GITB might provide specific therapeutic strategies for prevention and treatment of the infection in future. 相似文献